The Ogle Laboratory
Faculty > Brenda M. Ogle
Brenda M. Ogle
Assistant Professor, Biomedical Engineering (primary);
Material Science Program (affiliate)
ogle@wisc.edu
Organ System/Disease Focus
Cardiovascular Disease
Aligned Research Focus
Directed differentiation of stem cells to cells of the cardiovascular system; Advanced screening methods for stem cell.
Research Description
The mission of our research program is to investigate the mechanisms of stem cell differentiation, especially in the context of the cardiovascular system. Driven by this mission, we also seek to generate new technologies that advance stem cell biology and promote translation of stem cell research into clinical practice. Research highlights include:
- Discovery: Established that fusion between stem cells and mature cells facilitates survival and differentiation of stem cells. This finding expanded fundamental models of stem cell differentiation.
- Technology: Devised unique methods for inducing and tracking cell fusion in vitro and in vivo. These approaches allow testing of long-standing hypotheses related to the role(s) of stem cell fusion in health and disease, thus fueling a greater appreciation for this poorly-understood cell fate process.
- Discovery: Established that intrinsic fluorescence of metabolites of stem cells predictably identifies differentiation state.
- Technology: Developed a high throughput flow cytometry system capable of reliably measuring intrinsic fluorescence of stem cells and their progeny in a high-throughput way and of purifying populations based on these intrinsic biomarkers. This approach allows non-invasive purification of stem cell populations for experimental or clinical therapeutic intervention.
Selected References
Lin HP, Vincenz C, Kerppola T and Ogle BM. Bimolecular complementation analysis of eukaryotic fusion products. Biology of the Cell 102:525-537. 2010.
Kouris, NA, Squirrell, JM, Jung, JP, Pehlke, CA, Hacker, T, Eliceiri, KW, and Ogle BM. A non-denatured, non-crosslinked collagen matrix to deliver stem cells to the heart. Regenerative Medicine 6(5):569-582. 2011.
Buschke DG, Squirrell JM, Fong J, Eliceiri KW and Ogle BM. Cell death, noninvasively assessed by intrinsic fluorescence intensity of NADH, is a predictive indicator of functional differentiation of embryonic stem cells. Biology of the Cell, In Press.
Buschke DG, Resto P, Schumacher N, Cox B, Tallavajhula A, Vivekanandan A, Eliceiri KW, Williams J and Ogle BM. Microfluidic sorting of microtissues. Biomicrofluidics, 6:014116. 2012
Kouris NA, Schaefer JA, Hatta M, Kawaoka Y, Kamp TJ and Ogle BM. Directed fusion of mesenchymal stem cells with cardiomyocytes via VSV-G facilitates stem cell programming. Stem Cells International, In Press.